Research Article
MicroRNA Expression in Salivary Supernatant of Patients with Pancreatic Cancer and Its Relationship with ZHENG
Table 1
RT primers for amplification of the human mature miRNAs used in validation of microRNA microarray results.
| | Name | RT primer | Product length (bp) |
| |
miR-16 | F primer 5′-CGCGCTAGCAGCACGTAAATA-3′ | 71 | | R primer 5′-GTGCAGGGTCCGAGGT-3′ | | Probe 5′-TTCGCACTGGATACGACCGCCAA-3′ |
| miR-17
| F primer 5′-CGGCGGCAAAGTGCTTACAG-3′ | 73 | | R primer 5′-GTGCAGGGTCCGAGGT-3′ | |
Probe 5′-TTCGCACTGGATACGACCTACCTGCA-3′ |
| miR-21
| F primer 5′-GCGGCGGCTAGCTTATCAGAC-3′ | 74 | | R primer 5′-GTGCAGGGTCCGAGGT-3′ | | Probe 5′-TTCGCACTGGATACGACTCAACATCAG-3′ |
| miR-181a
| F primer 5′-GCCCGAACATTCAACGCTGT-3′ | 72 | | R primer 5′-GTGCAGGGTCCGAGGT-3′ | | Probe 5′-TTCGCACTGGATACGACACTCACCG-3′ |
| miR-181b
| F primer 5′-CGCGCAACATTCATTGCTGT-3′ | 66 | | R primer 5′-GTGCAGGGTCCGAGGT-3′ | | Probe 5′-TTCGCACTGGATACGACACCCAC-3′ |
| miR-196a
| F primer 5′-CGGCTTTGGCACTAGCACATT-3′ | 71 | | R primer 5′-GTGCAGGGTCCGAGGT-3′ | | Probe 5′-TTTGCGCACTGGATACGACAGCAA-3′ |
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