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BioMed Research International
Volume 2014, Article ID 758283, 8 pages
Research Article

Development of New Method for Simultaneous Analysis of Piracetam and Levetiracetam in Pharmaceuticals and Biological Fluids: Application in Stability Studies

1Faculty of Pharmacy, Federal Urdu University Arts, Science and Technology, Karachi 75300, Pakistan
2Faculty of Pharmacy, Ziauddin University, Karachi 75600, Pakistan
3Department of Chemistry, University of Karachi, Karachi 75270, Pakistan
4Department of Environmental Sciences, Federal Urdu University Arts, Science and Technology, Karachi 75300, Pakistan
5Faculty of Medicine, Ziauddin University, Karachi, Pakistan
6Department of Pharmacognosy, Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, University of Karachi, Karachi 75270, Pakistan

Received 5 February 2014; Accepted 4 June 2014; Published 8 July 2014

Academic Editor: Ali I. Abdalla

Copyright © 2014 Farhan Ahmed Siddiqui et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


RP-HPLC ultraviolet detection simultaneous quantification of piracetam and levetiracetam has been developed and validated. The chromatography was obtained on a Nucleosil C18 column of , 10 m, dimension. The mobile phase was a (70 : 30 v/v) mixture of 0.1 g/L of triethylamine and acetonitrile. Smooth flow of mobile phase at 1 mL/min was set and 205 nm wavelength was selected. Results were evaluated through statistical parameters which qualify the method reproducibility and selectivity for the quantification of piracetam, levetiracetam, and their impurities hence proving stability-indicating properties. The proposed method is significantly important, permitting the separation of the main constituent piracetam from levetiracetam. Linear behavior was observed between 20 ng/mL and 10000 ng/mL for both drugs. The proposed method was checked in bulk drugs, dosage formulations, physiological condition, and clinical investigations and excellent outcome was witnessed.