Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2014, Article ID 759791, 10 pages
http://dx.doi.org/10.1155/2014/759791
Research Article

Metabolic Effects of Hypoxia in Colorectal Cancer by 13C NMR Isotopomer Analysis

1Biophysics Unit, IBILI, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal
2Centre of Investigation on Environment, Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, 3001-301 Coimbra, Portugal
3Life Sciences Department, Faculty of Sciences and Technology, University of Coimbra, 3000-456 Coimbra, Portugal
4Faculty of Sciences and Technology, University of Coimbra, 3000-456 Coimbra, Portugal
5Radiotherapy Department, CHUC, 3000-075 Coimbra, Portugal
6Laboratory of Biochemical Genetics (CNC/UC), Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal
7Center for Neuroscience and Cell Biology (CNC), 3004-517 Coimbra, Portugal

Received 28 February 2014; Accepted 27 May 2014; Published 1 July 2014

Academic Editor: Zhiqiang Meng

Copyright © 2014 Ana M. Abrantes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. O. Warburg, “Origin of cancer cells,” Oncologia, vol. 9, no. 2, pp. 75–83, 1956 (German). View at Google Scholar
  2. S. Weinhouse, O. Warburg, D. Burk, and A. L. Schade, “On respiratory impairment in cancer cells,” Science, vol. 124, no. 3215, pp. 267–272, 1956. View at Publisher · View at Google Scholar · View at Scopus
  3. Y. Zhao, E. B. Butler, and M. Tan, “Targeting cellular metabolism to improve cancer therapeutics,” Cell Death and Disease, vol. 4, no. 3, article e532, 2013. View at Publisher · View at Google Scholar · View at Scopus
  4. C. V. Dang, “Links between metabolism and cancer,” Genes & Development, vol. 26, no. 9, pp. 877–890, 2012. View at Publisher · View at Google Scholar
  5. M. Talekar, S. R. Boreddy, A. Singh, and M. Amiji, “Tumor aerobic glycolysis: new insights into therapeutic strategies with targeted delivery,” Expert Opinion on Biological Therapy, 2014. View at Publisher · View at Google Scholar
  6. R. Xu, H. Pelicano, Y. Zhou et al., “Inhibition of glycolysis in cancer cells: a novel strategy to overcome drug resistance associated with mitochondrial respiratory defect and hypoxia,” Cancer Research, vol. 65, no. 2, pp. 613–621, 2005. View at Google Scholar · View at Scopus
  7. S. Ros and A. Schulze, “Balancing glycolytic flux: the role of 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatases in cancer metabolism,” Cancer & Metabolism, vol. 1, article 8, 2013. View at Publisher · View at Google Scholar
  8. E. Roudier and A. Perrin, “Considering the role of pyruvate in tumor cells during hypoxia,” Biochimica et Biophysica Acta, vol. 1796, no. 2, pp. 55–62, 2009. View at Publisher · View at Google Scholar · View at Scopus
  9. K. J. Williams, B. A. Telfer, R. E. Airley et al., “A protective role for HIF-1 in response to redox manipulation and glucose deprivation: implications for tumorigenesis,” Oncogene, vol. 21, no. 2, pp. 282–290, 2002. View at Publisher · View at Google Scholar · View at Scopus
  10. M. Achison and T. R. Hupp, “Hypoxia attenuates the p53 response to cellular damage,” Oncogene, vol. 22, no. 22, pp. 3431–3440, 2003. View at Publisher · View at Google Scholar · View at Scopus
  11. H. L. Kotze, E. G. Armitage, K. J. Sharkey, J. W. Allwood, W. B. Dunn, and K. J. Williams, “A novel untargeted metabolomics correlation-based network analysis incorporating human metabolic reconstructions,” BMC Systems Biology, vol. 7, article 107, 2013. View at Google Scholar
  12. Y.-A. Wen, P. D. Stevens, M. L. Gasser, R. Andrei, and T. Gao, “Downregulation of PHLPP expression contributes to hypoxia-induced resistance to chemotherapy in colon cancer cells,” Molecular and Cellular Biology, vol. 33, no. 22, pp. 4594–4605, 2013. View at Google Scholar
  13. B. Garcia-Barreno, T. Delgado, B. Akerlind-Stopner, E. Norrby, and J. A. Melero, “Location of the epitope recognized by monoclonal antibody 63G on the primary structure of human respiratory syncytial virus G glycoprotein and the ability of synthetic peptides containing this epitope to induce neutralizing antibodies,” Journal of General Virology, vol. 73, no. 10, pp. 2625–2630, 1992. View at Publisher · View at Google Scholar · View at Scopus
  14. I. F. Duarte, J. Marques, A. F. Ladeirinha et al., “Analytical approaches toward successful human cell metabolome studies by NMR spectroscopy,” Analytical Chemistry, vol. 81, no. 12, pp. 5023–5032, 2009. View at Publisher · View at Google Scholar · View at Scopus
  15. S. L. Pereira, J. Ramalho-Santos, A. F. Branco, V. A. Sardão, P. J. Oliveira, and R. A. Carvalho, “Metabolic remodeling during H9c2 myoblast differentiation: relevance for in vitro toxicity studies,” Cardiovascular Toxicology, vol. 11, no. 2, pp. 180–190, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. M. D. Basson, I. M. Modlin, and J. A. Madri, “Human enterocyte (Caco-2) migration is modulated in vitro by extracellular matrix composition and epidermal growth factor,” Journal of Clinical Investigation, vol. 90, no. 1, pp. 15–23, 1992. View at Publisher · View at Google Scholar · View at Scopus
  17. M. D. Peterson and M. S. Mooseker, “Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2,” Journal of Cell Science, vol. 102, no. 3, pp. 581–600, 1992. View at Google Scholar · View at Scopus
  18. E. M. Chance, S. H. Seeholzer, K. Kobayashi, and J. R. Williamson, “Mathematical analysis of isotope labeling in the citric acid cycle with applications to 13C NMR studies in perfused rat hearts,” Journal of Biological Chemistry, vol. 258, no. 22, pp. 13785–13794, 1983. View at Google Scholar · View at Scopus
  19. D. H. Williamson, P. Lund, and H. A. Krebs, “The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver,” Biochemical Journal, vol. 103, no. 2, pp. 514–527, 1967. View at Google Scholar · View at Scopus
  20. L. Suardet, A. C. Gaide, J. M. Calmès et al., “Responsiveness of three newly established human colorectal cancer cell lines to transforming growth factors β1 and β21,” Cancer Research, vol. 52, no. 13, pp. 3705–3712, 1992. View at Google Scholar · View at Scopus
  21. R. A. Carvalho, T. B. Rodrigues, P. Zhao, F. M. H. Jeffrey, C. R. Malloy, and A. D. Sherry, “A 13C isotopomer kinetic analysis of cardiac metabolism: influence of altered cytosolic redox and [Ca2+]o,” The American Journal of Physiology—Heart and Circulatory Physiology, vol. 287, no. 2, pp. H889–H895, 2004. View at Publisher · View at Google Scholar · View at Scopus
  22. A. M. Abrantes, M. E. S. Serra, A. C. Gonçalves et al., “Hypoxia-induced redox alterations and their correlation with 99mTc-MIBI and 99mTc-HL-91 uptake in colon cancer cells,” Nuclear Medicine and Biology, vol. 37, no. 2, pp. 125–132, 2010. View at Publisher · View at Google Scholar · View at Scopus