The Low Chamber Pancreatic Cancer Cells Had Stem-Like Characteristics in Modified Transwell System: Is It a Novel Method to Identify and Enrich Cancer Stem-Like Cells?
Comparison of the expression level of stem cell related genes and proteins in the three groups cells. (a) CD24, Oct4, and ESA genes expression in the lower chamber cells, bulk Panc-1 cells, and the upper chamber cells were detected by RT-PCR. (b) Expressions of CD24, Oct4, and ESA proteins in the lower chamber cells and Panc-1 cells were detected by Western blot. Data were normalized to β-actin levels. Experiments were repeated three times with similar data. L represents the lower chamber cells; C represents the bulk pancreatic cells; U represents the upper chamber cells (*, **, and ***).
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