Figure 6: Confocal imaging of gold nanorod uptake in 1321N1 human brain astrocytoma cells after 12-hour exposure to 36 μg/mL. Green coloring corresponds to the gold nanorods. The image effectively demonstrates that nanorods can be internalized into glioblastoma cells, including in the nucleus. Future investigation may elucidate the impact of internalized particles compared to nonspecific tissue-accumulated particles with regard to specificity in LITT treatments [24].