The Study of the Frequency Effect of Dynamic Compressive Loading on Primary Articular Chondrocyte Functions Using a Microcell Culture System
Figure 2
(a) Experimental quantification of the generated compressive strain by capturing the action images of micropillar using a high-speed CCD. The compressive strain (%) is measured by the equation: (deformation length)/ (height of 3D cell culture construct; the photograph (I))100%. Photographs (II)-(III) showed the observed deformation lengths of 0.45 mm () and 0.93 mm () with the corresponding compressive strain of 19.6% and 40.4%, respectively. (b) The quantitative relationship between the compressive strain and the pneumatic pressure applied under different operating frequency conditions (0.5, 1.0, and 2.0 Hz). (c) Evaluation of long-term stability of the generated compressive strain (lower and higher level: 20% and 40%, resp.) under the operating frequencies of 0.5, 1.0, and 2.0 Hz.
