Minocycline Enhances the Effectiveness of Nociceptin/Orphanin FQ during Neuropathic Pain
Figure 1
Repeated minocycline administration diminished the development of neuropathic pain and enhanced the effectiveness of N/OFQ. The response to N/OFQ was measured 20 and 40 minutes after administration by the von Frey test (a) and 30 and 50 minutes after administration by the cold plate test (b). Minocycline (MC; 30 mg/kg; i.p.) was administered intraperitoneally preemptively 16 h and 1 h before CCI and then repeatedly twice daily for 7 days. Vehicle-treated and minocycline-treated rats received intrathecal N/OFQ (2.5; 5 μg/5 μL) one hour after the last morning administration on day 7 after CCI. The data are presented as the mean response ± SEM. (8–16 rats per group). The results of the experiments were statistically evaluated using one-way analyses of variance (ANOVA). The differences between the treatment groups throughout the study were further analyzed with Bonferroni’s post hoc tests. , , and indicate significant differences compared with vehicle-treated CCI-exposed rats; and indicate significant differences between vehicle-treated CCI-exposed rats that received a single dose of N/OFQ and minocycline-treated CCI-exposed rats that received a single dose of N/OFQ. The dotted line is a value for naïve animals (von Frey test 25.8 g; cold plate test 29.7s).
