High-Throughput Functional Screening of Steroid Substrates with Wild-Type and Chimeric P450 Enzymes
Visualization of how wild-type CYP1A enzymes differentiate steroidal substrates. Left panel, MDS configuration plot based on the ratio model and characterized by a stress index of 0.016. Each point represents the main activity observed for each one of the steroidal substrates tested in this work except for mifepristone which is represented in this plot by two points (triangles). The solid triangle represents the main mifepristone (RU-486) hydroxylase activity with all CYP1A enzymes; the open triangle represents mifepristone N-demethylase activity. Right panel, dendrogram deduced from the correlation matrix used to build the MDS configuration plot. The dendrogram was built by the Ward method based on the dissimilarity matrix.