BioMed Research International / 2014 / Article / Tab 1

Research Article

High-Throughput Functional Screening of Steroid Substrates with Wild-Type and Chimeric P450 Enzymes

Table 1

Alignment of amino acid sequences of the J-helix of the four parental wild-type CYP1A enzymes studied in this work.

Mm CYP1A1341-PRVQRKIQEEL-351
Hs CYP1A1337-...........-347
Hs CYP1A2337-.EI.....K..-347
Oc CYP1A2337-.RR.....E..-347
       ▲      

HS: Homo sapiens, Mm: Mus musculus, and Oc: Oryctolagus cuniculus. The solid triangles indicate the charged residue discussed in the text; a dot represents an amino acid residue identical to that of Mm CYP1A1 sequence.