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BioMed Research International
Volume 2014, Article ID 765426, 6 pages
http://dx.doi.org/10.1155/2014/765426
Research Article

Development and Characterization of Liposomal Doxorubicin Hydrochloride with Palm Oil

1Department of Pharmacy, Faculty of Medicine, University of Malaya, 51200 Kuala Lumpur, Malaysia
2Faculty of Pharmacy, Cyberjaya University College of Medical Sciences, 63000 Cyberjaya, Selangor, Malaysia
3Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 141176, Iran

Received 17 December 2013; Revised 11 February 2014; Accepted 17 February 2014; Published 27 March 2014

Academic Editor: Paolo Colombo

Copyright © 2014 Bahareh Sabeti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The usage of natural products in pharmaceuticals has steadily seen improvements over the last decade, and this study focuses on the utilization of palm oil in formulating liposomal doxorubicin (Dox). The liposomal form of Dox generally minimizes toxicity and enhances target delivery actions. Taking into account the antiproliferative and antioxidant properties of palm oil, the aim of this study is to design and characterize a new liposomal Dox by replacing phosphatidylcholine with 5% and 10% palm oil content. Liposomes were formed using the freeze_thaw method, and Dox was loaded through pH gradient technique and characterized through in vitro and ex vivo terms. Based on TEM images, large lamellar vesicles (LUV) were formed, with sizes of 438 and 453 nm, having polydispersity index of 0.21 ± 0.8 and 0.22 ± 1.3 and zeta potentials of about −31 and −32 mV, respectively. In both formulations, the entrapment efficiency was about 99%, and whole Dox was released through 96 hours in PBS (pH = 7.4) at 37°C. Comparing cytotoxicity and cellular uptake of LUV with on MCF7 and MDA-MBA 231 breast cancer cell lines indicated suitable uptake and lower IC50 of the prepared liposomes.