A Functional Interplay between 5-Lipoxygenase and μ-Calpain Affects Survival and Cytokine Profile of Human Jurkat T Lymphocyte Exposed to Simulated Microgravity
Figure 1
Effect of simulated microgravity on μ-calpain expression and activity in Jurkat T cells. (a) RT-qPCR analysis of μ-calpain gene expression in Jurkat cells exposed to simulated microgravity (sim-μg) at 37°C for 48 hours. Gene levels were normalized to the housekeeping GAPDH and expressed as percentage of 1 g cells considered as control, set to 100 (b). Western blot analysis of protein expression in Jurkat cells treated as in (a). (c) ELISA analysis of μ-calpain protein content in Jurkat cells treated as in (a). Results are expressed as percentage of 1 g cells considered as control, set to 100 (= 9.48 ± 0.50 ng/per μg protein) (d) μ-calpain activity analysis in Jurkat cells treated as in (a) in absence (−) or in presence of 5 μM of calpastatin. Results are expressed as percentage of 1 g cells considered as control, set to 100 (= 66.26 ± 3.65 pmol/min per mg protein). (e) DNA fragmentation in Jurkat cells exposed to simulated microgravity for 48 hours in absence (−) or in presence of 10 μM E64D. Values are expressed as percentage of 1 g cells considered as control. denotes versus 1 g cells; denotes versus sim-μg cells.
