A Functional Interplay between 5-Lipoxygenase and μ-Calpain Affects Survival and Cytokine Profile of Human Jurkat T Lymphocyte Exposed to Simulated Microgravity

Figure 2

Effect of simulated microgravity on cytokine profile of Jurkat T cells. Cells were exposed (sim-μg) or not exposed (1 g) to simulated microgravity at 37°C for 48 hours and cytokine synthesis (gray bars) and release (black bars) were analyzed as reported in Section 2.6. Results are reported as percentage of 1 g cells set to 100. For synthesis, 100% of IL-2 = 0.27 ± 0.01  ; 100% of IL-4 = 0.34 ± 0.02  ; 100% of LIF = 0.22 ± 0.02  ; 100% of IL-6 = 0.18 ± 0.01  ; 100% of IL-10 = 0.34 ± 0.02  ; 100% of INF-γ  0.25 ± 0.02  . For release, 100% of LIF = 0.42 ± 0.03  ; 100% of IL-2 = 6.6 ± 0.5 pg/mL; 100% of IL-4 = 1.2 ± 0.1 pg/mL; 100% of IL-6 = 20 ± 2 pg/mL; 100% of IL-10 = 2.5 ± 0.3 pg/mL; 100% INF-γ = 12.2 ± 0.1 pg/mL. denotes versus 1 g cells; denotes versus 1 g cells.