A Functional Interplay between 5-Lipoxygenase and μ-Calpain Affects Survival and Cytokine Profile of Human Jurkat T Lymphocyte Exposed to Simulated Microgravity

Table 1

Time-dependent effect of simulated microgravity on apoptotic markers in Jurkat T cells exposed to simulated microgravity (sim-g) or kept at normal gravity (1g).

Parameter	1g	4-hour sim-g	24-hour sim-g	48-hour sim-g
DNA fragmentation	100 8	107 8	270	342
Cytochrome c release (cytosol/mitochondria ratio)	100 9	111 7	347	450

Results are expressed as percentage of 1g cells set to 100. For DNA fragmentation, 100% = 0.300 0.030 A_405 nm; for cytochrome c release, 100% = 0.074 0.005. denotes 0.01 versus 1g cells; denotes 0.001 versus 1g cells.