A Functional Interplay between 5-Lipoxygenase and μ-Calpain Affects Survival and Cytokine Profile of Human Jurkat T Lymphocyte Exposed to Simulated Microgravity

Table 3

Effect of 5-LOX inhibition on Jurkat T cells exposed for 48 hours to simulated microgravity (sim-g) or kept at normal gravity (1g).

Parameter	1g	sim-g	sim-g + 10 M AA861
DNA fragmentation	100 10	342 ±	250 ±
Cytochrome c release (cytosol/mitochondria ratio)	100 9	450 ±	230 ±
Calpain activity	100 ± 11	177 ±	31 ±
IL-2 protein content	100 ± 9	67 ±	93 ±
INF- protein content	100 ± 9	179 ±	120 ±

Values are reported as percentage of relative control set to 100. For DNA fragmentation, 100% = 0.30 ± 0.03 A_405 nm; for cytochrome c release, 100% = 0.074 ± 0.005; for calpain activity, 100% = 66.26 ± 3.65 pmol/min per mg protein; for IL-2 synthesis, 100% = 0.27 0.01 A_405 nm; for INF- synthesis, 100% = 0.25 ± 0.02 A_405 nm. denotes = < 0.05 versus 1g cells; denotes < 0.01 versus 1g cells; denotes < 0.001 versus 1g cells; denotes < 0.01 versus sim-g cells.