Glucagon Effects on ³H-Histamine Uptake by the Isolated Guinea-Pig Heart during Anaphylaxis

Table 3

Obtained values of histamine, NO, O₂⁻, and H₂O₂ in the samples of coronary venous effluent collected: before glucagon perfusion, during glucagon perfusion (sample collected during 4-5 min of glucagon perfusion) but before ovalbumin application, during ovalbumin application, that is, in the first 60 s of anaphylaxis in the presence of glucagon, 1 min after ovalbumin application, in the presence of glucagon, and 5 min after ovalbumin application, in the absence of glucagon.

	Histamine; (𝜇M)	NO; (nmol/mL)	O2^−; (nmol/mL)	H2O2; (nmol/mL)
Before glucagon perfusion	4.8±0.42×10^−8	2.49±0.12	26.64±1.47	2.16±0.19
During glucagon perfusion and before ovalbumin application	4.98±0.2×10^−8	5.69^∗±0.3	21.08±2.67	2.19±0.26
During ovalbumin application (anaphylaxis) in the presence of glucagon	6.9^∗±0.25×10^−8	4.77^∗±0.33	29.57±1.36	1.74±0.11
One minute after ovalbumin application in the presence of glucagon	7.38^∗±0.28×10^−8	3.1±0.19	30.58±2.1	2.31±0.12
Five minutes after ovalbumin application in the absence of glucagon	5.18±0.2×10^−8	4.7±1.0	31.62±2.8	2.71±0.24

Data are presented as mean ± SE, 𝑛=8.
*Significantly different values (𝑃<0.05).