Research Article

SIRT1 Inhibition Affects Angiogenic Properties of Human MSCs

Figure 3

Effects of SIRT1 inhibition on migration and capillary tube network formation. (a) hMSCs were treated with sirtinol 100 μM (black bars) or equivalent concentration of DMSO (white bars) for 24, 48, and 72 h. (b) sh-Sirt1-hMSCs migration (black bars) compared to control (white bars) at 24, 48, and 72 h. Each histogram indicates the % of migrated cells measured at different times. Statistical differences were denoted with when is compared to control. (c) Tubule formation promoted by hMSCs treated with sirtinol (50 and 100 μM) (upper panel) and tubular structures of sh-Sirt1 infected hMSCs plated on Matrigel compared to control (lower panel). Magnification 10x. Scale bar = 200 μm. (d) Tubule formation was quantified by counting the number of branch points of the capillary network. Data are expressed as mean ± SD. denotes statistical differences when is compared to control.