Obligatory Role for Endothelial Heparan Sulphate Proteoglycans and Caveolae Internalization in Catestatin-Dependent eNOS Activation
Figure 4
CST induces proteoglycan-dependent Cav1 internalization in GFP-Cav1 transfected BAE-1 cells. (a) Representative time course of the fluorescence intensity in single BAE-1 cells transfected with Cav-1-GFP and stimulated with H:ase + CST or with CST alone. Subpanels 1 and 2: confocal pseudocolor images of CST-treated BAE-1 cells from the correspondent time points indicated by the arrows, showing membrane (1) and cytosolic (2) localizations of GFP-Cav1 after CST treatment. (b) Bar graphs representing the percent variation with respect to control of the fluorescent GFP-Cav1 signal in the different experimental conditions (CST = %; H:ase + CST = −%; sets of experiments, 3 fields/sample, about 40 cells/fields; ). (c) Bar graph representing the percent variation with respect to control of the fluorescent GFP-Cav1 signal during CST 5 nM treatment, respectively, in plasma membrane and in cytosol (membrane = −%; cytosol %; cells; ).
