Obligatory Role for Endothelial Heparan Sulphate Proteoglycans and Caveolae Internalization in Catestatin-Dependent eNOS Activation

Figure 5

CST prevents Cav1/eNOS colocalization in a PI3K-dependent manner. (a) Confocal immunofluorescence images of BAE-1 cells showing the typical membrane localization of Cav1 (red staining) and eNOS (green staining). After CST administration, Cav1 dissociated from eNOS and staining diffused in the cytosol. This effect was reversed by Wm pretreatment. Immunofluorescence detection was carried out using Alexa Fluor 488 anti-mouse for total eNOS and Cy3 anti-rabbit for Cav1. Scale bar: 20 μm. (b) Bar graph representing Pearson correlation values relative to Cav1 and eNOS staining in each experimental condition (control = 0.84±0.09; CST = −0.07±0.05; Wm + CST = 0.69±0.18; 𝑛=8 sets of experiments, 3 fields/sample, about 30 cells/fields; 𝑃<0.05).

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