Obligatory Role for Endothelial Heparan Sulphate Proteoglycans and Caveolae Internalization in Catestatin-Dependent eNOS Activation

Figure 6

Both proteoglycans and caveolae are required to allow CST-dependent eNOS phosphorylation. (a) Typical Western blot experiment showing that CST-induced P^Ser1179eNOS was reduced by both M𝛽CD (5 mM, 30 min) and H:ase (2 U/mL). (b) P^Ser1179eNOS/𝛽-actin ratio of densitometric values from Western blots (%P^Ser1179eNOS/𝛽-actin: control = 3.59±1; CST = 18.81±1.57; M𝛽CD + CST = 3.48±1.5, H:ase + CST = 1.63±0.41; 𝑛=3; 𝑃<0.05).

(a)

(b)