Comparative Biomechanical and Microstructural Analysis of Native versus Peracetic Acid-Ethanol Treated Cancellous Bone Graft
Figure 5
(a)–(f) Histological analysis of native cancellous bone (NCB) and peracetic acid-ethanol (PE)-treated cancellous bone allograft (CBG). Fluorescence microscopic image of cross sections from NCB (a) and PE-treated CBG (b) after Alizarin staining (scale bar = 1000 μm). Micrographs of NCB (c) and PE-treated CBG (d) after hematoxylin and eosin staining demonstrating connective tissue in the NCB sample (scale bar = 50 μm). Asterisks and plus sign denote cancellous bone and connective tissue, respectively. Arrow indicates crescent-shaped lamellae running parallel to the bone marrow interface; no tissue is visible in PE-treated CBG (d). Histologic sections of NCB (e) and PE-treated CBG (f) after Giemsa staining (scale bar = 50 μm). Arrows correspond to osteocytes colored in dark purple. The fine cellular structures of osteocytes with dendritic processes and nuclei of NCB are clearly visible. In contrast, sections of PE-treated CBG demonstrate pores from bone forming osteocytes with their rested nuclei with remnant cellular structures after PE treatment.
