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BioMed Research International
Volume 2014, Article ID 787034, 11 pages
Research Article

Cross-Comparison of Leaching Strains Isolated from Two Different Regions: Chambishi and Dexing Copper Mines

1School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan 410083, China
2Key Laboratory of Biohydrometallurgy, Ministry of Education, Changsha 410083, China

Received 28 April 2014; Revised 23 July 2014; Accepted 12 August 2014; Published 16 November 2014

Academic Editor: Paula Loureiro Paulo

Copyright © 2014 Baba Ngom et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Material Table S1: This table is a selected biochemical and physiological characteristics of the new isolated strains. As expected, the new isolates are all mesophilic chemolitotroph; their optimal condition for growth range between 30 and 35°C, and they use ferrous ions (At. ferrooxidans, L. ferriphilum BN) and inorganic sulfur compounds (At. thiooxidans) as energy source and dioxide carbon as sole carbon source. None of them could use organic substrates, but they were differently affected by the presence of carbohydrates, such as glucose, sucrose, yeast extract, peptone, tryptone soya broth and organic acids (acetic and propionic acid). For instance, strains of At. ferrooxidans were inhibited by glucose and sucrose but stimulated by peptone and tryptone soya broth at concentration lower than 0.05% (wt/v), while that of L. ferriphilum were inhibited by all these carbon substances.

The isolates were also tested in high level ferric and copper ions concentrations. As expected, L. ferriphilum BN showed a higher tolerance to ferric ions (500 mM) than At. ferrooxidans FOX1 (300mM) and At. thiooxidans ZMB (200 mM). For copper tolerance also BN showed a higher copper tolerance than FOX1 (60 against 30 mM).

Figure S1: (it’s Figure S1 and not Figure S2) This figure shows the response of iron oxidizers under different concentration of peptone and tryptone (0, 0.5 and 1.0 gL-1). Leptospirillum ferriphilum strains were inhibited with the increasing concentration of tryptone and peptone. In contrast, Acidithiobacillus ferrooxidans strains showed different responses according to the concentration of peptone and tryptone. The latter promoted the iron oxidation rate of At. ferrooxidans strains at concentration lower than 0.5 gL-1; while they inhibited the oxidation of ferrous ions at 1.0 gL-1.

  1. Supplementary Material