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BioMed Research International
Volume 2014, Article ID 787365, 10 pages
http://dx.doi.org/10.1155/2014/787365
Research Article

18FDG, [18F]FLT, [18F]FAZA, and 11C-Methionine Are Suitable Tracers for the Diagnosis and In Vivo Follow-Up of the Efficacy of Chemotherapy by miniPET in Both Multidrug Resistant and Sensitive Human Gynecologic Tumor Xenografts

1Department of Nuclear Medicine, University of Debrecen, P.O. Box 63, Nagyerdei Körút 98, Debrecen 4012, Hungary
2Department of Biophysics and Cell Biology, University of Debrecen, Nagyerdei Körút 98, Debrecen 4012, Hungary
3Department of Dermatology, University of Debrecen, Nagyerdei Körút 98, Debrecen 4012, Hungary
4Department of Surgery and Operative Techniques, Faculty of Dentistry, University of Debrecen, Nagyerdei Körút 98, Debrecen 4012, Hungary
5Department of Obstetrics and Gynecology, University of Debrecen, Nagyerdei Körút 98, Debrecen 4012, Hungary

Received 16 January 2014; Revised 14 August 2014; Accepted 28 August 2014; Published 18 September 2014

Academic Editor: Yi-Xiang Wang

Copyright © 2014 György Trencsényi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Expression of multidrug pumps including P-glycoprotein (MDR1, ABCB1) in the plasma membrane of tumor cells often results in decreased intracellular accumulation of anticancer drugs causing serious impediment to successful chemotherapy. It has been shown earlier that combined treatment with UIC2 anti-Pgp monoclonal antibody (mAb) and cyclosporine A (CSA) is an effective way of blocking Pgp function. In the present work we investigated the suitability of four PET tumor diagnostic radiotracers including 2-[18F]fluoro-2-deoxy-D-glucose (18FDG), 11C-methionine, 3′-deoxy-3′-[18F]fluorothymidine (18F-FLT), and [18F]fluoroazomycin-arabinofuranoside (18FAZA) for in vivo follow-up of the efficacy of chemotherapy in both Pgp positive (Pgp+) and negative (Pgp) human tumor xenograft pairs raised in CB-17 SCID mice. Pgp+ and Pgp A2780AD/A2780 human ovarian carcinoma and KB-V1/KB-3-1 human epidermoid adenocarcinoma tumor xenografts were used to study the effect of the treatment with an anticancer drug doxorubicin combined with UIC2 and CSA. The combined treatment resulted in a significant decrease of both the tumor size and the accumulation of the tumor diagnostic tracers in the Pgp+ tumors. Our results demonstrate that 18FDG, 18F-FLT, 18FAZA, and 11C-methionine are suitable PET tracers for the diagnosis and in vivo follow-up of the efficacy of tumor chemotherapy in both Pgp+ and Pgp human tumor xenografts by miniPET.