High Prevalence of Panton-Valentine Leukocidin (PVL) Genes in Nosocomial-Acquired Staphylococcus aureus Isolated from Tertiary Care Hospitals in Nepal
Figure 1
(a) PCR-RFLP of coa and spa amplified products of PVL-positive MRSA (12 isolates, 48–198) and PVL-negative MRSA (4 isolates, 3–41) digested by HaeII; M = 1 kb DNA ladder. PVL-negative MRSA isolates cluster under a single restriction pattern, while PVL-positive MRSA isolates demonstrate clustering under two distinct restriction types: type I (48–120, 154–161, and 193–198) and type II (147, 177). The isolates were obtained from two tertiary care hospitals. (b) Corresponding dendogram of the PCR-RFLP of PVL-positive and PVL-negative MRSA isolates.
