High Prevalence of Panton-Valentine Leukocidin (PVL) Genes in Nosocomial-Acquired Staphylococcus aureus Isolated from Tertiary Care Hospitals in Nepal
Figure 2
(a) PCR-RFLP of coa and spa amplified products of PVL-positive MSSA (14 isolates, 14–194) and PVL-negative MSSA (3 isolates, 79–113) digested by HaeII; M = 1 kb DNA ladder. PVL-positive MSSA isolates 16 and 47 cluster under one restriction type and isolates 14, 31, and 185 cluster under another. The remaining nine PVL-positive MSSA isolates and all three PVL-negative MSSA isolates exist as singletons. (b) Corresponding dendogram of the PCR-RFLP of PVL-positive and PVL-negative MSSA isolates.
