In Search of the Active Metabolites of an Anticancer Piperazinedione, TW01003, in Rats
Figure 2
Typical high pressure liquid chromatograph of untreated urine of pig (a); pig urine after TW01003 administration (b); and sulfatase-treated pig urine after TW01003 dosing (c). Parent compound TW01003, TW01003 sulfate, and internal standard HPW044X11 were identified with retention times at 9.17 min, 16.59 min, and 25.85 min, respectively. TW01003 glucuronide with a retention time at 12.5 min was barely visible.
