Effects of Freshwater Pollution on the Genetics of Zebra Mussels (Dreissena polymorpha) at the Molecular and Population Level

Table 1

Primer set used for microsatellite analysis.

Locus	Primer sequence (5′-3′)
A6	F: GTT TCT TTG CCG GTC TAA TAA TAG AGT TAA C
	R: GTG ATT GTG TAT CTG CTA TAA ACC
B6	F: GTT TCT TCG TGT GCT CAT GTT TCC TCC
	R: CGT TGT TCA AGC AAT AAG AAA GAC
B9	F: GTT TCT TTT GAC AAT ATC CTG TCT AAT G
	R: GCG TGT GTT TTT GAA ACG TG
C5	F: GTT TCT TGC ACT GTC AAC GTC ACA CTT TTG
	R: CCT TGC TAA CAG CTC GGT TGT ATC
Dpol9	F: M13(-18)TGG TTG ATG CAG TGA CCC TA
	R: TGT CGC TTG ATC CAT GTT TT
Dpol19	F: M13(-18)GCA TTC CAT CAA AAA CAC AGA T
	R: GAT CAA CAC CAA AGT TCG TTT C

The primers are based on Naish and Boulding [22], Astanei et al. [23], and Thomas et al. [24]. We slightly modified the primer pairs of the loci A6, B6, B9, and C5 (Naish and Boulding [22]; Astanei et al. [23]).