Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System

Table 1

Bacterial strains and plasmids used in this study.

Genotype and characteristics	Genotype and characteristics	Source/reference
1711-4	aEPEC O51:H40; wild-type	Gomes et al., 2004 [21]
1711-4 ΔescN	escN::zeo (Zeo^r)	This study
1711-4 ΔescN (pEscN)	1711-4 ΔescN carrying pEscN (Zeo^r, Clo^r)	This study
pEscN	pACYC184 carrying the escN gene from tEPEC E2348/69 strain	Gauthier et al., 2003 [6]
pKOBEG-Apra^r	Derivative (Apra^r) of pKOBEG plasmid encoding the; λ phage red operon	Chaveroche et al., 2000 [22]
MC4160-malTΔ224::zeo-(F+)	Source of zeocin cassette	Gift from J. M. Ghigo

rMeans that the strain is resistant to that antibiotic.