Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System

Table 2

Primers used for construction and verification of mutation.

Designation	Primer sequence
Allelic exchange
ESCN.zeo-5	5′-TGGGAATAATATCGAACTTAAAGTATTAGGAACGGTAAATGGTCATCGCTTGCATTAGAAAG-3′
ESCN.zeo-3	5′-CGCTCTGCTTTTACGAATAGATAAAATTCTGTCCAACATATTCAGAATGATGCAGAGATGTAAG-3′
Verification
ESCN.verf-5	5′-TCAGGCGCTATGTGAAGAAA-3′
ESCN.verf-3	5′-TACGCCTGCTTAGAGGCAAT-3′

aUnderlined bases correspond to 5′ and 3′ regions of the Sh ble gene, which encodes zeocin resistance.