In Vitro Chronic Administration of ERbeta Selective Ligands and Prostate Cancer Cell Growth: Hypotheses on the Selective Role of 3beta-Adiol in AR-Positive RV1 Cells

Table 2

Target protein	Primary antibody	Secondary antibody
ERbeta (all)	Ab288; (Abcam, Cambridge, UK); 1 : 500 dilution	WesternDot 625 detection kits; (Life Technologies Italia, Monza, Italy); 1 : 2000 dilution
AR	Sc816; (Santa Cruz Biotechnology, Santa Cruz, CA, USA); 1 : 400 dilution	HRP-conjugated anti-rabbit; (Santa Cruz Biotechnology, Santa Cruz, CA, USA); 1 : 2000 dilution
PTEN	ab32199; (Abcam, Cambridge, UK); 1 : 1000 dilution	HRP-conjugated anti-rabbit; (Santa Cruz Biotechnology, Santa Cruz, CA, USA); 1 : 8000 dilution
p21	05-345; (Millipore, Billerica, MA, USA); 1 : 1000 dilution	HRP-conjugated anti-mouse; (Santa Cruz Biotechnology, Santa Cruz, CA, USA); 1 : 5000 dilution
Tubulin	T9026; (Sigma-Aldrich, Monza, Italy); 1 : 2000 dilution	HRP-conjugated anti-mouse; (Santa Cruz Biotechnology, Santa Cruz, CA, USA); 1 : 8000 dilution
Beta-actin	Sc1616; (Santa Cruz Biotechnology, Santa Cruz, CA, USA); 1 : 4000 dilution	HRP-conjugated anti-goat; (Santa Cruz Biotechnology, Santa Cruz, CA, USA); 1 : 4000 dilution