Connexin 43 Expression on Peripheral Blood Eosinophils: Role of Gap Junctions in Transendothelial Migration
Figure 3
Dye transfer between eosinophils and epithelial/endothelial cells. Eosinophils were cocultured with A549 airway epithelial cells or HMVEC-L for 3 h. In each case one of the cell types was labeled and the other unlabeled. Unlabeled cells (eosinophils in (a) and (c), A549 cells in (b) and HMVEC-L in (d)) were gated and analyzed for evidence of dye transfer from the labeled cells. A representative experiment (from 4 to 6 experiments) is shown for each condition. Numbers in the graph indicate % of positive cells. Conditions: (a) transfer from labeled A549 cells to unlabeled eosinophils, (b) transfer from labeled eosinophils cells to unlabeled A549 cells, (c) transfer from labeled HMVEC-L cells to unlabeled eosinophils, and (d) transfer from labeled eosinophils to unlabeled HMVEC-L cells.
