Connexin 43 Expression on Peripheral Blood Eosinophils: Role of Gap Junctions in Transendothelial Migration
Figure 4
Effect of Cx inhibitors on dye transfer and transendothelial migration. (a) Unlabeled HMVEC-L cells were cocultured with labeled eosinophils (Eosin) or HMVEC-L (HMVEC-L). The graph shows % decrease in mean fluorescent intensity of unlabeled HMVEC-L in the presence of various concentrations of 18-a-glycyrrhetinic acid compared to mean fluorescent intensity in the absence of 18-a-glycyrrhetinic acid (). (b) Residual dye in labeled eosinophils or HMVEC-L following coculture with unlabeled HMVEC-L in the presence or absence of 18-a-glycyrrhetinic acid. The graph shows “% change mean fluorescent intensity (amount of dye)” in labeled eosinophils or HMVEC-L in the presence of various concentrations of 18-a-glycyrrhetinic acid compared to dye content in the absence of 18-a-glycyrrhetinic acid (). (c) Effect of various concentrations of 18-a-glycyrrhetinic acid on eosinophil transmigration through endothelial monolayers grown on transwells without fibronectin. (d) Effect of 18-a-glycyrrhetinic acid in transmigration of eosinophils or neutrophils through endothelial monolayers grown on transwells coated with FN.
