Integrated Autofluorescence Characterization of a Modified-Diet Liver Model with Accumulation of Lipids and Oxidative Stress
Figure 3
Examples of AF distribution patterns from unfixed, unstained liver tissue sections from control (a) and MCD diet administered rats (b–d), at and after 10 sec of continuous exposure to excitation light. Irradiation results in the persistence of the bright fibrous network in control (a′). In MCD diet samples (b′–d′), excitation light results in the disappearance of the greenish signal rising from the vesicles (white arrows), in parallel with the persistence of fibrous structures (light blue arrows). Bright granules are also observable, in particular at 9 weeks of MCD diet administration, ascribable to lipofuscin like material (yellow arrows) due to their yellowish emission color and resistance to irradiation. Bar: 160 μm.
