Solanine Induces Mitochondria-Mediated Apoptosis in Human Pancreatic Cancer Cells

Table 1

Primer pairs used in RT-PCR in vitro and in vivo.

Genes	Primers (5′-3′)	BP
In vitro
Bax	Forward ACAAAGATGGTCACGGTCTGCC	242
	Reverse ACCAAGAAGCTGAGCGAGTGTC
Bcl-2	Forward GCTCT TCAGG GACGG GGT	166
	Reverse GACAG CCAGG AGAAA TCAAA CAG
P53	Forward TTCCG AGAGC TGAAT GAGGC	435
	Reverse TTTTT ATGGC GGGAG GTAGA CT
Cytc	Forward AGACA TGGAG ACCAA AATCA AGAAC	132
	Reverse CTCCT TTAGC GGTCA TTGCC
Smac	Forward AGCTG GAAAC CACTT GGATGA	138
	Reverse GAATG TGATT CCTGG CGGTTA
MMP-2	Forward AATGC CATCC CCGAT AACC	397
	Reverse GCTCA GCAGC CTAGC CAGTC
MMP-9	Forward GGGGG AAGAT GCTGC TGTT	440
	Reverse AGCGG TCCTG GCAGA AATAG
GAPDH	Forward GTCTT CACCA CCATG GAGAA	267
	Reverse ATCCA CAGTC TTCTG GGTGG
In vivo
Bax	Forward TGGCAGCTGACATGTTTTCTGAC	195
	Reverse CGTCCCAACCACCCTGGTCT
Bcl-2	Forward GTCATGTGTGTGGAGAGCGT	144
	Reverse GCCGTACAGTTCCACAAAGG
Caspase-3	Forward CAGACAGTGGAACTGACGAT	152
	Reverse TTTCAGCATGGCGCAAAGTG
GAPDH	Forward GGTGGAAGGTCGGTGTGAACG	234
	Reverse CTCGCTCCTGGAAGATGGTG