Research Article

Barcoding Melting Curve Analysis for Rapid, Sensitive, and Discriminating Authentication of Saffron (Crocus sativus L.) from Its Adulterants

Figure 1

Melting curves of saffron and its adulterants. Saffron, 7 adulterants, and 1 : 1 mix of authentic saffron with adulterants were amplified in triplicate by the primer pair psbAF/trnHR and performed melting curve analysis by a real-time PCR system. Mixture means mix of saffron with the corresponding adulterant in one PCR reaction.