Barcoding Melting Curve Analysis for Rapid, Sensitive, and Discriminating Authentication of Saffron (Crocus sativus L.) from Its Adulterants
Table 3
Predicted melting temperature () of saffron and its adulterants from the sequence based on public database NCBI.
Species
Predicted (°C)
trnH-psbA
matK
rbcL
trnL-F
ITS2
trnH-psbA
Size (bp)
GC%
(°C)
Crocus sativus
82.76
Not mentioned
83.54
90.84
83.38
609
37
81.92
Dendranthema morifolium
84.41
85.68
83.62
89.04
80.82
436
27
79.62
Calendula officinalis
83.69
87.23
83.94
90.79
81.31
476
27
79.92
Carthamus tinctorius
84.31
86.04
84.30
89.86
80.66
441
27
80.10
Nelumbo nucifera
84.28
86.12
89.73
89.52
80.11
359
27
80.58
Hemerocallis fulva
82.74
85.51
83.50
Not mentioned
83.95
669
35
84.78
Zea mays
Not mentioned
81.03
82.50
89.19
84.46
616
38
84.33
Daucus carota
84.11
86.12
83.71
88.73
83.86
315
38
81.08
Melting temperature of trnL-F, trnH-psbA, rbcL, matK, and ITS2 barcodes was predicted by an online OligoCalc program, amplicon size, and GC content was also calculated and the experiment was present. represent the melting temperature from Bar-MCA result.
