Characterization of a Mycobacterium avium subsp. avium Operon Associated with Virulence and Drug Detoxification

Figure 3

RT-PCR of lprG, p55, and the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (gapdh) were used as the internal control. MAA wild type and MAAΔlprG cDNA were obtained using random hexamers for conversion of mRNA. Two different specific primers pairs were used to amplify lprG (lprG-1 and lprG-2), p55 (p55-1 and p55-2), and gapdh (gapdh 1 and gapdh 2). The introduction of a Hyg resistance cassette in the coding sequence of lprG of the MAA D4ER strain induced a polar effect on the expression of the downstream gene p55.