Inhibition of MMP-2 Expression with siRNA Increases Baseline Cardiomyocyte Contractility and Protects against Simulated Ischemic Reperfusion Injury
Figure 3
Effect of MMP-2 siRNA transfection on MMP-2 expression in isolated cardiomyocytes. (a) Efficiency of siRNA transfection and MMP-2 protein levels measured by immunocytochemistry. Scale bar, 50 μm. (b) Measurement of MMP-2 gelatinolytic activity by zymography. (c) MMP-2 protein level. (a) cells from 3 different hearts per group; heart isolates per group for MMP-2 activity (b), in (c) heart isolates per group, versus aerobic control.