Inhibition of MMP-2 Expression with siRNA Increases Baseline Cardiomyocyte Contractility and Protects against Simulated Ischemic Reperfusion Injury

Figure 5

Effect of MMP-2 siRNA transfection on the level of the contractile proteins MLC1 and MLC2 (a), the formation of the complex between MMP-2 and MLC1 or MLC2 (b), and cardiomyocyte contractility (c). As a protein loading control the tubulin level was measured. Control cells were transfected with scrambled siRNA. 𝑛=4--6 heart preparation isolations per group. 𝑛=11 per group for contractility measurement and 𝑛=4--6 for measurement of protein levels. ^∗𝑃<0.05 versus control.

(a)

(b)

(c)