Evaluation of Cytotoxic and Antimicrobial Effects of Two Bt Cry Proteins on a GMO Safety Perspective
Figure 1
(a) Electrophoretic profile of the heterologous gene expression of Cry8Ka5 protein in polyacrylamide gel (12.5%) under reducing and denaturing conditions. Lane 1: molecular weight markers. Lane 2: peak not retained from the chromatography on the Ni-NTA resin. Lane 3: peak retained from the chromatography on the Ni-NTA resin eluted with 20 mM imidazole. (b) Electrophoretic profile of the heterologous gene expression of Cry1Ac protein in polyacrylamide gel (12.5%) under reducing and denaturing conditions. Lane 1: molecular weight marker; Lane 2: protoxin Cry1Ac not dialyzed; Lane 3: protoxin Cry1Ac dialyzed; Lane 4: toxin Cry1Ac after trypsinization and dialysis.
