Evaluation of Cytotoxic and Antimicrobial Effects of Two Bt Cry Proteins on a GMO Safety Perspective

Table 4

Cytotoxicity evaluation of Cry8Ka5, Cry1Ac, and protein and nonprotein controls against Artemia sp. nauplii after 24 h exposure.

Samples	Concentrations (𝜇g/mL)	Mortality^a (%)	LC50^b (𝜇g/mL)
Cry8Ka5	1,000	66.67	755.11 ± 86.88; (623.93–978.60)^c
	500	26.67
	250	6.67
	125	0
Cry1Ac	1,000	10	>1,000
Bovine serum albumin	1,000	3.33	>1,000
Artificial seawater	—	0	—
Temephos^d	—	—	0.16

aValues are means of triplicate for each sample. The standard deviations were less than 5% of means; ^bSample concentration capable of killing 50% of Artemia sp. nauplii (confidence limit of 95%); ^c(lower limit, upper limit); ^dTaken from Souza et al. [26].