Age and SPARC Change the Extracellular Matrix Composition of the Left Ventricle

Table 1

Quantitative values (normalized weighted spectra) for the identified extracellular proteins (𝑛=4  per group, values are mean ± SEM).

Identified Proteins	Molecular weight	Young WT	Young KO	Middle WT	Middle KO	Old WT	Old KO	Accession number
Secreted proteins
Annexin A2	39 kDa	0	0	0	0	0.5±0.5	0.5±0.5	gi∣12849385
Apolipoprotein O	21 kDa	0	0.8±1.0	0	0	0	0	gi∣123122452
Collagen type I alpha-1	138 kDa	10.0±0.4	7.8±2.6	12.5±2.6	7.0±0.8	10.5±1.2	6.0±1.8	gi∣34328108
Collagen type I alpha-2	130 kDa	6.0±0.7	3.5±1.3	9.5±1.6	3.5±1.3	9.3±1.4	7.0±0.6	gi∣111120329
Collagen type III alpha-1	139 kDa	0.3±0	0.3±0	1.5±0.8	0.5±0	1.3±0	0.8±0	gi∣74184771
Collagen type IV alpha-2	167 kDa	1.5±0.6	0.8±0.6	3.8±0.6	2.3±0.6	4.3±0.8	3.0±0	gi∣556299
Collagen type VI alpha-1	108 kDa	7.8±0.6	8.8±0.5	7.5±1.2	7.8±1.9	6.0±1.1	6.3±0.6	gi∣6753484
Collagen type VI alpha-2	110 kDa	4.0±0.9	6.0±1.2	7.8±1.5	9.5±1.4	11.0±0.7	9.5±1.7	gi∣22203747
Collagen type VI alpha-3	287 kDa	41.8±6	46.3±7	45.0±7	51.5±7	51.3±5	48.0±4	gi∣148708135
Collagen type XV	140 kDa	0	0	0	0.3±0.3	0	0	gi∣11037306
Collagen type XXIV alpha-1	176 kDa	0	0	0.3±0.3	0	0	0	gi∣116326001
Fibrillin-1	312 kDa	0	0.3±0.3	0	0	0	0.5±0.5	gi∣118197277
Fibrinogen, alpha	87 kDa	0.3±0	0	0	1.0±0.6	3.3±1.7	3.0±1.3	gi∣148683476
Fibrinogen, beta	55 kDa	0.0	0	0.3±0	0.5±0.5	1.3±0.9	0.5±0.5	gi∣33859809
Fibrinogen, gamma	49 kDa	0.0	0	0	0	0.5±0.5	0	gi∣148683478
Laminin alpha-2	344 kDa	0.8±0	1.5±0.7	0.5±0.5	0.3±0.3	0	0.3±0.3	gi∣117647249
Laminin beta-1	204 kDa	1.0±0.7	0.5±0.3	0.3±0.3	0.3±0.3	1.0±0.6	0.3±0.3	gi∣148704971
Laminin beta-2	197 kDa	0.3±0.3	1.5±0.9	0.5±0.5	1.5±0.6	0.5±0.3	1.3±0.5	gi∣31982223
Laminin gamma-1	179 kDa	1.8±0.5	1.0±0.4	2.0±0.8	5.5±1.2	2.3±0.9	2.5±0.6	gi∣148707495
Nidogen-1	137 kDa	1.0±0.6	1.3±0.5	0	0.3±0.3	0.5±0.5	0.8±0.3	gi∣171543883
Perlecan	470 kDa	4.8±0.5	4.8±0.3	3.5±1.0	5.5±1.2	5.8±0.8	6.8±1.3	gi∣183979966
Prelamin-A/C	74 kDa	0.3±0	0.8±0.5	0.3±0	0	0	0	gi∣162287370
Serum albumin	69 kDa	0	0	0	1.0±0.5	0.3±0.3	0	gi∣163310765
Troponin I, cardiac muscle	24 kDa	15.8±0.9	13.5±1.2	11.8±2.1	14.3±0.9	10.8±2.6	9.8±2.0	gi∣6678393
Troponin T2, cardiac	32 kDa	0.8±0.5	1.0±0.7	0.5±0.5	0	0	0	gi∣148707615
Von Willebrand factor A domain-containing protein 8	213 kDa	2.3±0.6	3.5±0.6	1.8±0.4	2.0±0.5	3.0±0.5	3.0±0.8	gi∣226958579
Cell membrane proteins
Alpha-sarcoglycan	43 kDa	0	0	0	0	0	0.3±0.3	gi∣2411510
Aminopeptidase	103 kDa	0	0.5±0.5	0.5±0.5	1.5±1.0	0	0.5±0.5	gi∣1184161
ATP1a1 protein	108 kDa	5.3±1.9	6.8±2.9	4.3±3.0	6.5±3.8	2.0±0.8	1.3±0.8	gi∣16307541
ATP-binding cassette (ALD) 3	75 kDa	0	0	0	0	0	0	gi∣14318642
ATP-binding cassette (MDR/TAP) 8	78 kDa	0.8±0.5	0.0	0.5±0.5	0	0	0	gi∣148671187
EH domain-containing protein 4	61 kDa	2.0±0.7	1.5±0.9	2.8±0.9	4.0±0.4	1.5±0.5	2.8±0.3	gi∣31981592
Guanine nucleotide-binding protein, subunit beta-2	37 kDa	0	1.3±0.6	0.5±0.2	0.5±0.3	0.5±0.3	0	gi∣13937391
Guanosine diphosphate dissociation inhibitor 2	53 kDa	0	0	0	1.0±1	0	0	gi∣148700276
Neutral cholesterol ester hydrolase 1	46 kDa	4.5±0.3	3.0±0.4	0	0.3±0.3	0	0.5±0.5	gi∣30520239
PDZ and LIM domain protein 5	24 kDa	2.5±0.5	3.3±0.6	1.5±0.6	2.0±0.4	0.5±0.3	0.8±0.8	gi∣300069034
Perilipin-4	139 kDa	3.5±1.2	5.0±0.8	4.8±0.6	6.0±2.0	8.0±2.0	6.8±1.6	gi∣157041252
Peroxisomal membrane protein 20	17 kDa	2.3±0.3	3.5±0.5	1.3±1.3	1.5±0.3	1.5±0.5	1.5±0.9	gi∣6746357
Platelet glycoprotein 4	53 kDa	0	1.3±1.3	0.5±0.5	0	0	0	gi∣74151899
Protease, serine 15	109 kDa	0.5±0.5	0	0	0	0	0	gi∣148706233
Sodium/calcium exchanger 1	108 kDa	0.5±0.5	0.8±0.8	0	0	0	0	gi∣119120890
Sorbin and SH3 domain-containing protein 1	83 kDa	3.5±1.6	4.0±0.9	6.3±0.6	5.0±0.9	6.5±0.9	5.3±1.3	gi∣78000154
Transglutaminase	77 kDa	0	0	0	0	0	0.3±0.3	gi∣201941
Tripartite motif-containing protein 72	53 kDa	6.5±1.3	8.0±0.8	10.0±0.7	10.3±1.8	7.8±1.3	11.3±1.1	gi∣121247302