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BioMed Research International
Volume 2014, Article ID 819093, 7 pages
http://dx.doi.org/10.1155/2014/819093
Research Article

The Influence of Standardized Valeriana officinalis Extract on the CYP3A1 Gene Expression by Nuclear Receptors in In Vivo Model

1Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, Marii Magdaleny 14, 61-861 Poznan, Poland
2Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland
3Department of Rehabilitation, Poznan University of Medical Sciences, 28 Czerwca 1956, 61-545 Poznan, Poland
4Department of Pharmacology, Poznan University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland
5Department of Pharmacology and Biotechnology, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland
6Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Science, Marii Magdaleny 14, 61-861 Poznan, Poland

Received 19 February 2014; Revised 28 August 2014; Accepted 29 August 2014; Published 11 September 2014

Academic Editor: Stelvio M. Bandiera

Copyright © 2014 Anna Bogacz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Valeriana officinalis is one of the most popular medicinal plants commonly used as a sedative and sleep aid. It is suggested that its pharmacologically active compounds derived from the root may modulate the CYP3A4 gene expression by activation of pregnane X receptor (PXR) or constitutive androstane receptor (CAR) and lead to pharmacokinetic herb-drug interactions. The aim of the study was to determine the influence of valerian on the expression level of CYP3A1 (homologue to human CYP3A4) as well as nuclear receptors PXR, CAR, RXR, GR, and HNF-4. Male Wistar rats were given standardized valerian extract (300 mg/kg/day, p.o.) for 3 and 10 days. The expression in liver tissue was analyzed by using real-time PCR. Our result showed a decrease of CYP3A1 expression level by 35% () and 37% (), respectively. Moreover, Valeriana exhibited statistically significant reduction in RXR (approximately 28%) only after 3-day treatment. We also demonstrated a decrease in the amount HNF-4 by 22% () and 32% (), respectively. In case of CAR, the increase of expression level by 46% () was noted. These findings suggest that Valeriana officinalis extract can decrease the CYP3A4 expression and therefore may lead to interactions with synthetic drugs metabolized by this enzyme.