Abarema cochliacarpos Extract Decreases the Inflammatory Process and Skeletal Muscle Injury Induced by Bothrops leucurus Venom
Figure 5
Light microscopy of mouse EDL muscle 24 h after perimuscular injection of B. leucurus venom: effect of A. cochliacarpos extract (EAc). Longitudinal sections stained with hematoxylin and eosin: (a) PSS; (b) venom (1.0 mg/kg); (c) venom + dexamethasone (2 mg/kg, i.v); (d) venom + EAc 100 mg/kg; (e) venom + EAc 200 mg/kg; and (f) venom + EAc 400 mg/kg ( per group). In panel (a), panoramic view of control muscle showing normal multinucleated myofibers with peripheral nuclei (arrow) and no morphological changes. Panels (b), (d), and (e) show necrotic myofibers in different stages of degeneration (arrowheads), edema between the fibers (stars) and intense inflammatory infiltrate (asterisks) that reaches the internal portions of the muscle. In (c) and (f), observe preserved muscle fibers with peripheral nuclei (arrows). We can see some inflammatory cells (diagonal arrows) and small vacuoles (lozenges). Magnification: 40x.
