Tumor and Endothelial Cell Hybrids Participate in Glioblastoma Vasculature
Figure 4
Glioblastoma stem cell fusion with endothelial cells. (a) When GSC-GFP were cocultured with HUVEC-DsRed, we observed binucleated cell expressing both DsRed (A) and GFP (B) (merged in (C)) corresponding to hybrid cell and exhibiting endothelial phenotype as shown by CD31 immunostaining (D). (b) After hybrid cell selection by cell sorting, mononuclear hybrids expressing parental GFP (A) and DsRed (B) were observed (merged in (C)). Apoptotic body of died fused cell (arrow).
