Repeated Batch Fermentation Biotechnology for the Biosynthesis of Lipid and Gamma-Linolenic Acid by Cunninghamella bainieri 2A1

Table 4

Analysis of variance for the second-order polynomial model of lipid synthesis by Cunninghamella bainieri 2A1 using repeated batch cultivation at 30°C for 96 h.

Source	Polynomial coefficients	Sum of squares	DF	Mean square	𝐹 value	Prob > 𝐹
Model		18.27	9	2.03	134.41	<0.0001**
Intercept	4.55
𝑋1	0.051	0.026	1	0.026	1.72	0.2258
𝑋2	−0.15	0.24	1	0.24	15.70	0.0042**
𝑋3	1.28	16.49	1	16.49	1091.56	<0.0001**
𝑋^21	−0.18	0.085	1	0.085	5.61	0.0453*
𝑋^22	−0.35	0.34	1	0.34	22.22	0.0015**
𝑋^23	0.098	0.026	1	0.026	1.73	0.2253
𝑋1𝑋2	0.26	0.53	1	0.53	35.12	0.0004**
𝑋1𝑋3	−0.11	0.092	1	0.092	6.12	0.0385*
𝑋2𝑋3	−0.12	0.12	1	0.12	7.63	0.0246*
Residuals		0.12	8	0.015
Lack of fit		0.091	5	0.018	1.82	0.3305
Pure error		0.030	3	1.000𝐸−002

∗Statistically significant at 95% probability level.
∗∗Statistically significant at 99% of probability level.
𝑋₁: glucose concentration (g/L); 𝑋₂: ammonium tartrate (g/L); 𝑋₃: harvesting time (h). 𝑋²₁, 𝑋²₂, and 𝑋²₃: the quadratic terms; 𝑋₁𝑋₂, 𝑋₁𝑋₃, and 𝑋₂𝑋₃: the interaction terms.
𝑅²=0.9934.