Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2014, Article ID 832736, 9 pages
http://dx.doi.org/10.1155/2014/832736
Research Article

Generation of Insulin-Producing Cells from Human Bone Marrow-Derived Mesenchymal Stem Cells: Comparison of Three Differentiation Protocols

1Department of Biotechnology, Urology and Nephrology Center, Mansoura 35516, Egypt
2Department of Nephrology, Urology and Nephrology Center, Mansoura 35516, Egypt
3Department of Pathology, Urology and Nephrology Center, Mansoura 35516, Egypt
4Department of Immunology, Urology and Nephrology Center, Mansoura 35516, Egypt
5Zewail University of Science and Technology, 6th of October City, Giza 12588, Egypt
6Department of Urology, Urology and Nephrology Center, Mansoura 35516, Egypt

Received 9 January 2014; Revised 3 March 2014; Accepted 12 March 2014; Published 10 April 2014

Academic Editor: Aijun Wang

Copyright © 2014 Mahmoud M. Gabr et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. E. A. Ryan, B. W. Paty, P. A. Senior et al., “Five-year follow-up after clinical islet transplantation,” Diabetes, vol. 54, no. 7, pp. 2060–2069, 2005. View at Publisher · View at Google Scholar · View at Scopus
  2. R. Y. Calne, S. U. Gan, and K. O. Lee, “Stem cell and gene therapies for diabetes mellitus,” Nature Reviews Endocrinology, vol. 6, no. 3, pp. 173–177, 2010. View at Publisher · View at Google Scholar · View at Scopus
  3. N. Lumelsky, O. Blondel, P. Laeng, I. Velasco, R. Ravin, and R. McKay, “Differentiation of embryonic stem cells to insulin-secreting structures similar to pancreatic islets,” Science, vol. 292, no. 5520, pp. 1389–1394, 2001. View at Publisher · View at Google Scholar · View at Scopus
  4. H. Segev, B. Fishman, A. Ziskind, M. Shulman, and J. Itskovitz-Eldor, “Differentiation of human embryonic stem cells into insulin-producing clusters,” Stem Cells, vol. 22, no. 3, pp. 265–274, 2004. View at Google Scholar · View at Scopus
  5. E. Kroon, L. A. Martinson, K. Kadoya et al., “Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo,” Nature Biotechnology, vol. 26, no. 4, pp. 443–452, 2008. View at Publisher · View at Google Scholar · View at Scopus
  6. L. Denner, Y. Bodenburg, J. G. Zhao et al., “Directed engineering of umbilical cord blood stem cells to produce C-peptide and insulin,” Cell Proliferation, vol. 40, no. 3, pp. 367–380, 2007. View at Publisher · View at Google Scholar · View at Scopus
  7. M. F. Pittenger, A. M. Mackay, S. C. Beck et al., “Multilineage potential of adult human mesenchymal stem cells,” Science, vol. 284, no. 5411, pp. 143–147, 1999. View at Publisher · View at Google Scholar · View at Scopus
  8. M. M. Gabr, M. M. Sobh, M. M. Zakaria, A. F. Refaie, and M. A. Ghoneim, “Transplantation of insulin-producing clusters derived from adult bone marrow stem cells to treat diabetes in rats,” Experimental and Clinical Transplantation, vol. 6, no. 3, pp. 236–243, 2008. View at Google Scholar · View at Scopus
  9. M. M. Gabr, M. M. Zakaria, A. F. Refaie et al., “Insulin-producing cells from adult human bone marrow mesenchymal stem cells control streptozotocin-induced diabetes in nude mice,” Cell Transplantation, vol. 22, no. 1, pp. 133–145, 2013. View at Publisher · View at Google Scholar
  10. E. Hisanaga, K. Y. Park, S. Yamada et al., “A simple method to induce differentiation of murine bone marrow mesenchymal cells to insulin-producing cells using conophylline and betacellulin-delta4,” Endocrine Journal, vol. 55, no. 3, pp. 535–543, 2008. View at Publisher · View at Google Scholar · View at Scopus
  11. T. Tayaramma, B. Ma, M. Rohde, and H. Mayer, “Chromatin-remodeling factors allow differentiation of bone marrow cells into insulin-producing cells,” Stem Cells, vol. 24, no. 12, pp. 2858–2867, 2006. View at Publisher · View at Google Scholar · View at Scopus
  12. M. W. Pfaffl, “A new mathematical model for relative quantification in real-time RT-PCR,” Nucleic Acids Research, vol. 29, no. 9, article e45, 2001. View at Google Scholar · View at Scopus
  13. Y. Jiang, B. N. Jahagirdar, R. L. Reinhardt et al., “Pluripotency of mesenchymal stem cells derived from adult marrow,” Nature, vol. 418, no. 6893, pp. 41–49, 2002. View at Publisher · View at Google Scholar · View at Scopus
  14. D. Marappagounder, I. Somasundaram, S. Dorairaj, and R. J. Sankaran, “Differentiation of mesenchymal stem cells derived from human bone marrow and subcutaneous adipose tissue into pancreatic islet-like clusters in vitro,” Cellular and Molecular Biology Letters, vol. 18, no. 1, pp. 75–88, 2013. View at Publisher · View at Google Scholar
  15. R. S. Y. Wong, “Extrinsic factors involved in the differentiation of stem cells into insulin-producing cells: an overview,” Experimental Diabetes Research, vol. 2011, Article ID 406182, 15 pages, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. S. Dave, “Extrinsic factors promoting insulin producing cell-differentiation and insulin expression enhancement-hope for diabetics,” Current Stem Cell Research and Therapy, vol. 8, no. 6, pp. 471–483, 2013. View at Publisher · View at Google Scholar
  17. B. Soria, “In-vitro differentiation of pancreatic β-cells,” Differentiation, vol. 68, no. 4-5, pp. 205–219, 2001. View at Google Scholar · View at Scopus
  18. N. Z. Sun and H. Ji, “In vitro differentiation of human placenta-derived adherent cells into insulin-producing cells,” Journal of International Medical Research, vol. 37, no. 2, pp. 400–406, 2009. View at Google Scholar · View at Scopus
  19. I. Kojima and K. Umezawa, “Conophylline: a novel differentiation inducer for pancreatic β cells,” International Journal of Biochemistry and Cell Biology, vol. 38, no. 5-6, pp. 923–930, 2006. View at Publisher · View at Google Scholar · View at Scopus
  20. L. Li, M. Seno, H. Yamada, and I. Kojima, “Promotion of β-cell regeneration by betacellulin in ninety percent-pancreatectomized rats,” Endocrinology, vol. 142, no. 12, pp. 5379–5385, 2001. View at Publisher · View at Google Scholar · View at Scopus
  21. L. Li, M. Seno, H. Yamada, and I. Kojima, “Betacellulin improves glucose metabolism by promoting conversion of intraislet precursor cells to β-cells in streptozotocin-treated mice,” The American Journal of Physiology—Endocrinology and Metabolism, vol. 285, no. 3, pp. E577–E583, 2003. View at Google Scholar · View at Scopus
  22. R. Kitamura, T. Ogata, Y. Tanaka et al., “Conophylline and betacellulin-δ4: an effective combination of differentiation factors for pancreatic β cells,” Endocrine Journal, vol. 54, no. 2, pp. 255–264, 2007. View at Publisher · View at Google Scholar · View at Scopus
  23. V. M. Richon and J. P. O'Brien, “Histone deacetylase inhibitors: a new class of potential therapeutic agents for cancer treatment,” Clinical Cancer Research, vol. 8, no. 3, pp. 662–664, 2002. View at Google Scholar · View at Scopus
  24. K. Otoguro, R. Oiwa, Y. Iwai, H. Tanaka, and S. Omura, “Screening for new antitrichomonal substances of microbial origin and antitrichomonal activity of trichostatin A,” Journal of Antibiotics, vol. 41, no. 4, pp. 461–468, 1988. View at Google Scholar · View at Scopus
  25. S. Bonner-Weir, D. Deery, J. L. Leahy, and G. C. Weir, “Compensatory growth of pancreatic β-cells in adult rats after short-term glucose infusion,” Diabetes, vol. 38, no. 1, pp. 49–53, 1989. View at Google Scholar · View at Scopus
  26. A. Suzuki, H. Nakauchi, and H. Taniguchi, “Glucagon-like peptide 1 (1–37) converts intestinal epithelial cells into insulin-producing cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 9, pp. 5034–5039, 2003. View at Publisher · View at Google Scholar · View at Scopus
  27. X. H. Wu, C. P. Liu, K. F. Xu et al., “Reversal of hyperglycemia in diabetic rats by portal vein transplantation of islet-like cells generated from bone marrow mesenchymal stem cells,” World Journal of Gastroenterology, vol. 13, no. 24, pp. 3342–3349, 2007. View at Google Scholar · View at Scopus
  28. A. K. Chatterjee, J. Sieradzki, and H. Schatz, “Epidermal growth factor stimulates (pro-)insulin biosynthesis and 3H-thymidine incorporation in isolated pancreatic rat islets,” Hormone and Metabolic Research, vol. 18, no. 12, pp. 873–874, 1986. View at Google Scholar · View at Scopus
  29. L. Li, Z. Yi, M. Seno, and I. Kojima, “Activin A and betacellulin: effect on regeneration of pancreatic β-cells in neonatal streptozotocin-treated rats,” Diabetes, vol. 53, no. 3, pp. 608–615, 2004. View at Publisher · View at Google Scholar · View at Scopus
  30. H. Kolb and V. Burkart, “Nicotinamide in type I diabetes: mechanism of action revisited,” Diabetes Care, vol. 22, supplement 2, pp. B16–B20, 1999. View at Google Scholar · View at Scopus
  31. S. G. Straub, G. Shanmugam, and G. W. G. Sharp, “Stimulation of insulin release by glucose is associated with an increase in the number of docked granules in the β-cells of rat pancreatic islets,” Diabetes, vol. 53, no. 12, pp. 3179–3183, 2004. View at Publisher · View at Google Scholar · View at Scopus
  32. J. F. Dishinger, K. R. Reid, and R. T. Kennedy, “Quantitative monitoring of insulin secretion from single islets of langerhans in parallel on a microfluidic chip,” Analytical Chemistry, vol. 81, no. 8, pp. 3119–3127, 2009. View at Publisher · View at Google Scholar · View at Scopus
  33. S. M. Phadnis, M. V. Joglekar, M. P. Dalvi et al., “Human bone marrow-derived mesenchymal cells differentiate and mature into endocrine pancreatic lineage in vivo,” Cytotherapy, vol. 13, no. 3, pp. 279–293, 2011. View at Publisher · View at Google Scholar · View at Scopus
  34. Q. Shi, S. Luo, H. Jia et al., “Insulin-producing cells could not mimic the physiological regulation of insulin secretion performed by pancreatic β cells,” Nanoscale Research Letters, vol. 8, no. 1, pp. 90–97, 2013. View at Publisher · View at Google Scholar
  35. O. Karnieli, Y. Izhar-Prato, S. Bulvik, and S. Efrat, “Generation of insulin-producing cells from human bone marrow mesenchymal stem cells by genetic manipulation,” Stem Cells, vol. 25, no. 11, pp. 2837–2844, 2007. View at Publisher · View at Google Scholar · View at Scopus
  36. A. S. Boyd, D. C. Wu, Y. Higashi, and K. J. Wood, “A comparison of protocols used to generate insulin-producing cell clusters from mouse embryonic stem cells,” Stem Cells, vol. 26, no. 5, pp. 1128–1137, 2008. View at Publisher · View at Google Scholar · View at Scopus
  37. S. J. Kim, Y. S. Choi, E. S. Ko, S. Lim, C. Lee, and D. Kim, “Glucose-stimulated insulin secretion of various mesenchymal stem cells after insulin-producing cell differentiation,” Journal of Bioscience and Bioengineering, vol. 113, no. 6, pp. 771–777, 2012. View at Publisher · View at Google Scholar · View at Scopus
  38. Wei, J. Yang, W. Hou et al., “Insulin-producing cells derived from human embryonic stem cells: comparison of definitive endoderm- and nestin-positive progenitor-based differentiation strategies,” PLoS ONE, vol. 8, no. 8, Article ID e72513, 2013. View at Publisher · View at Google Scholar
  39. I. Ilie, R. Ilie, T. Mocan, F. Tabaran, C. Iancu, and L. Mocan, “Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic β cells via MIF pathway,” International Journal of Nanomedicine, vol. 3, no. 8, pp. 3345–3353, 2013. View at Publisher · View at Google Scholar