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BioMed Research International
Volume 2014, Article ID 836025, 9 pages
http://dx.doi.org/10.1155/2014/836025
Research Article

Targeting the Raft-Associated Akt Signaling in Hepatocellular Carcinoma

1Department of Pathology and Hepatology, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China
2First Hepatobiliary Surgery Center, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China
3Secondary Hepatobiliary Surgery Center, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China
4Beijing Institute of Infectious Diseases, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China

Received 29 April 2014; Revised 30 June 2014; Accepted 14 July 2014; Published 27 August 2014

Academic Editor: Jun Yong Park

Copyright © 2014 Yuan Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Caveolin-1 and flotillin-1 are considered as markers of lipid rafts which can be regarded as sorting platforms for targeted transport of transmembrane proteins and are involved in fundamental cellular events such as signal transduction, cell adhesion, lipid/protein sorting, and human cancer. We addressed caveolin-1 and flotillin-1 expression in 90 human hepatocellular carcinoma (HCC) and adjacent noncancerous tissues (ANT) samples by SDS-PAGE and immunoblotting with specific antibodies. Significant caveolin-1 and flotillin-1 overexpression was found in HCC tissues compared to ANT and was confirmed by immunohistochemistry. Raft-associated Akt signaling pathway components involved in the regulation of cell survival were altered by western blotting in HCC microdomain-enriched subcellular fractions purified from paired HCC and ANT samples. Our results demonstrated that the activity of raft-associated but not total membrane Akt determines its cellular functions. Lipid rafts differ in different types of tissues, which allows for the possibility of tissue-type-specific targeting for cell survival.