A Higher Frequency of CD4+CXCR5+ T Follicular Helper Cells in Adult Patients with Minimal Change Disease
Figure 6
Flow cytometry analysis of B cells. The relationship of the percentages of different subsets of T follicular helper cells (TFH) with B cells in MCD patients. PBMCs from MCD patients and HC were stained with anti-CD19, anti-CD38, anti-CD95, and anti-IgD. The cells were gated initially on living lymphocytes and then on CD19+ B cells. Subsequently, the frequency of CD19+IgD+CD27− B cells, CD19+IgD+CD27+ B cells, CD19+IgD−CD27+ B cells, CD19+IgD−CD27− B cells, CD19+CD86+ B cells, and CD19+CD95+ B cells was analyzed by flow cytometry. (a) Flow cytometry analysis, (b) quantitative analysis, and (c) the percentages of different subsets of T follicular helper (TFH) and B cells. Data shown are representative dot plug or expressed as the mean % of different subsets of B cells. The horizontal lines represent the median values.
