Human Umbilical Cord Perivascular Cells Exhibited Enhanced Migration Capacity towards Hepatocellular Carcinoma in Comparison with Bone Marrow Mesenchymal Stromal Cells: A Role for Autocrine Motility Factor Receptor
In vivo migration of BM-MSCs and HUCPVCs. CM-DiI and DiR prelabeled MSCs were i.v. injected in s.c. HuH7 tumor-bearing mice. At day 3 mice were sacrificed and organs were removed; lungs, livers, spleen (a) and tumors (c) were exposed to obtain FI. Images represent the radiant efficiency. Representative images are shown. (b) Total FI for injected BM-MSCs or HUCPVCs was calculated by measuring the region of interest (ROI) for all the tissues isolated and results were expressed as total radiant efficiency [p/s]/[μW/cm2]. . (d) Signal present in the isolated liver, spleen, lungs and tumors was represented as percentage of total signal for BM-MSCs or HUCPVCs-injected mice. versus BM-MSCs. (e) Microscopic analysis of transplanted CM-DiI-labeled MSCs (red signal indicated by arrows) and DAPI staining in frozen sections of tumors. ×200 magnification. (f) In vitro migration of MSCs to TCM derived from HuH7 or HC-PT-5 s.c. tumors. Bars represent the average of MSCs/field (10x) ± SEM from three representative visual fields. Results are representative of 3 independent experiments. versus BM-MSCs.
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