Biological Assessment of a Calcium Silicate Incorporated Hydroxyapatite-Gelatin Nanocomposite: A Comparison to Decellularized Bone Matrix

Figure 3

In vitro assessment of osteoblast activity on the material-coated dishes. (1) Focal adhesion assay of MC3T3 cells on the coated culture plate with no coating as control (a), HGCS (b), and DECBM (c) after 3 days of culture. MTS assay for MC3T3-E1 cell proliferation on the coated culture plate with DECBM, GEMOSIL-CS, and no coating as control on 1, 3, 5, and 7 days of culture (d). Mineralized nodules were detected by Alizarin Red staining after culturing MC3T3 cells with osteogenic media on the coated culture plate with no coating as control (e), HGCS (f), and DECBM (g) after 7, 14, and 21 days. Negative control was also tested as coated culture plate without cells for 21 days with osteogenic media.